Part:BBa_K4404004
cooC1, acsV, AWO_c10690 and AWO_c10700 from A. woodii
This is a fragment with a length of 4.12 kb, which was synthesised by de-novo-synthesis with the genomic background of Acetobacerium woodii. It consists of the genes cooC1, acsV, AWO_c10690 and AWO_c10700. cooC1 codes for an accessory protein of the Ni2+ insertion. acsV regenerates the corrinoid iron sulfur protein. AWO_c10690 and AWO_c10700 are putative proteins. All these genes are parts of the Wood-Ljungdahl pathway of acetogenic bacteria. The gene coding fragment is flanked by specific restriction sites and overhangs. Upstream it is flanked by a Sal1 restriction site and a 5 base pairs overhang. Downstream it is flanked by a Pac1 restriction site and a 5 base pairs overhang.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1561
Illegal EcoRI site found at 1887 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1561
Illegal EcoRI site found at 1887 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1561
Illegal EcoRI site found at 1887 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1561
Illegal EcoRI site found at 1887 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1561
Illegal EcoRI site found at 1887
Illegal AgeI site found at 66
Illegal AgeI site found at 1978
Illegal AgeI site found at 2575 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2942
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